Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat Sterol Regulatory Element-binding Protein 1 (also known as SREBP1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat SREBP1 antibody. SREBP1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat SREBP1 Antibody is added and binds to SREBP1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SREBP1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat SREBP1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm